Secretion of metalloproteinases by stimulated capillary endothelial cells. I. Production of procollagenase and prostromelysin exceeds expression of proteolytic activity.
作者： Z Werb , M J Banda , G S Herron , K Dwyer
DOI: 10.1016/S0021-9258(17)35859-3
关键词:
摘要: We have characterized the biosynthesis of two metalloproteinases, procollagenase and prostromelysin, by rabbit brain capillary endothelial cells (RBCE) means immunochemical, biosynthetic, functional assays. Unstimulated RBCE secreted no detectable metalloproteinases. Secretion both prostromelysin was induced within 6 h treating with 50 ng/ml 12-O-tetradecanoylphorbol-13-acetate. In treated cells, proenzymes accounted for up to 20% [35S]methionine-labeled proteins; about 15 micrograms each protein in 48 10(6) RBCE. Although approximately as much did fibroblasts, virtually enzyme activity could be measured RBCE-conditioned medium, even after activation trypsin or an organomercurial agent.
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