Purification and characterization of bovine dental pulp collagenase inhibitor.

摘要: Root pulps from bovine unerupted wisdom teeth produce a potent collagenase inhibitor together with latent when cultured in Eagle's minimal essential medium (Biochem. Int. 5, 763, 1982). The was purified more than 700-fold the explant using Con A-Sepharose, Ultrogel AcA 44 and DE-52 cellulose columns. It showed single band (MW = 36,000) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, but multiple bands basic (pH 8.3) polyacrylamide electrophoresis electrofocusing. is sialo-glycoprotein containing approx. 20% carbohydrate by weight its composition suggests that it contains complex-type oligosaccharides. electrophoretic heterogeneity of proved to be due attachment different numbers sialic acid residues. All SH groups were demonstrated exist as six disulfide linkages which might involved inhibitory activity. pulp does not combine collagen. addition activated resulted dose-dependent inhibition enzyme activity, interaction between tight enough for complex remain intact during filtration column chromatography. A rabbit antiserum prepared against inhibitor, immunoglobulin can completely abolish activity inhibitor.