Assay of plasma dehydroepiandrosterone and its sulphate by competitive protein binding.
作者： C.M. Andre , V.H.T. James
DOI: 10.1016/0009-8981(73)90465-8
关键词: Extraction (chemistry) 、 Binding properties 、 Sodium borohydride 、 Chromatography 、 Globulin 、 Paper chromatography 、 Dehydroepiandrosterone 、 Solvolysis 、 Chemistry 、 Plasma protein binding
摘要: Abstract A competitive protein-binding method for the assay of dehydroepiandrosterone and its sulphate in plasma is described. After extraction from plasma, solvolysis case sulphate, purified by paper chromatography transformed to 5-androstene-3β, 17β-diol reduction with sodium borohydride. The so formed prior assay. end-point uses binding property testosterone-binding globulin, which binds high affinity those steroids an unhindered 17β-hydroxyl group. reliability criteria terms precision, accuracy, sensitivity specificity have been evaluated.
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sci-hub.se 参考文章(13)
I. E. Bush, Methods of paper chromatography of steroids applicable to the study of steroids in mammalian blood and tissues Biochemical Journal. ,vol. 50, pp. 370- 378 ,(1952) , 10.1042/BJ0500370
Shlomo Burstein, Seymour Lieberman, Hydrolysis of Ketosteroid Hydrogen Sulfates by Solvolysis Procedures Journal of Biological Chemistry. ,vol. 233, pp. 331- 335 ,(1958) , 10.1016/S0021-9258(18)64759-3
DARREL MAYES, CHARLES A. NUGENT, Determination of plasma testosterone by the use of competitive protein binding. The Journal of Clinical Endocrinology and Metabolism. ,vol. 28, pp. 1169- 1176 ,(1968) , 10.1210/JCEM-28-8-1169
J TOWNSEND, V JAMES, A semi-automated fluorimetric procedure for the determination of plasma corticosteroids. Steroids. ,vol. 11, pp. 497- 511 ,(1968) , 10.1016/S0039-128X(68)80064-9
HORTENSE M. GANDY, RALPH E. PETERSON, Measurement of Testosterone and 17-Ketosteroids in Plasma by the Double Isotope Dilution Derivative Technique1 The Journal of Clinical Endocrinology and Metabolism. ,vol. 28, pp. 949- 977 ,(1968) , 10.1210/JCEM-28-7-949
Armando de la Pena, Joseph W. Goldzieher, Separation of free and bound steroid in the competitive protein binding assay for testosterone: accuracy and reproducibility. Steroids. ,vol. 18, pp. 195- 201 ,(1971) , 10.1016/0039-128X(71)90026-2
J. B. BROWN, R. D. BULBROOK, F. C. GREENWOOD, AN EVALUATION OF A CHEMICAL METHOD FOR THE ESTIMATION OF OESTRIOL, OESTRONE AND OESTRADIOL-17β IN HUMAN URINE Journal of Endocrinology. ,vol. 16, pp. 41- 48 ,(1957) , 10.1677/JOE.0.0160041
R. Hampl, L. Stárka, Determination of dehydroepiandrosterone sulfate in human plasma by competitive protein binding Clinica Chimica Acta. ,vol. 34, pp. 77- 83 ,(1971) , 10.1016/0009-8981(71)90069-6
Robert L. Rosenfield, A competitive protein binding method for the measurement of unconjugated and sulfate-conjugated dehydroepiandroster one in peripheral plasma☆ Steroids. ,vol. 17, pp. 689- 696 ,(1971) , 10.1016/0039-128X(71)90084-5
TATSUO KATO, RICHARD HORTON, Studies of Testosterone Binding Globulin The Journal of Clinical Endocrinology and Metabolism. ,vol. 28, pp. 1160- 1168 ,(1968) , 10.1210/JCEM-28-8-1160