The lymphocyte transformation test in allergic contact dermatitis: New opportunities
作者： Amy Popple , Jason Williams , Gavin Maxwell , Nichola Gellatly , Rebecca J. Dearman
DOI: 10.3109/1547691X.2015.1008656
关键词: Contact allergens 、 Skin sensitization 、 Lymphocyte transformation 、 Allergic contact dermatitis 、 Immune system 、 Patch testing 、 Medicine 、 Immunology 、 Antigen 、 Ex vivo
摘要: Allergic contact dermatitis (ACD) is driven by the activation and proliferation of allergen-specific memory T-lymphocytes currently diagnosed patch testing with a selected panel chemical allergens. The lymphocyte transformation test (LTT) can be used to monitor ex vivo T-lymphocyte responses antigens, including LTT not viewed as being an alternative testing, but it does seek reflect experimentally skin sensitization specific chemicals. based on stimulation in vitro antigen-driven proliferation. That is, exposure culture primed relevant antigen delivered appropriate configuration will provoke secondary response that reflects acquisition sensitization. technical aspects this utility approach for investigation immune allergens humans are reviewed here, particular emphasis further development refinement protocol. An important potential application may provide basis characterizing those have greatest influence sensitizing potency considered some detail.
manchester.ac.uk参考文章(95)
Kazunari Sugita, Kenji Kabashima, Yu Sawada, Sanehito Haruyama, Manabu Yoshioka, Tomoko Mori, Miwa Kobayashi, Kouetsu Ogasawara, Yoshiki Tokura, Blocking of CTLA-4 on lymphocytes improves the sensitivity of lymphocyte transformation tests in a patient with nickel allergy European Journal of Dermatology. ,vol. 22, pp. 268- 269 ,(2012) , 10.1684/EJD.2012.1641
Nick J. Gonchoroff, Jerry A. Katzmann, Rose M. Currie, Elizabeth L. Evans, David W. Houck, Bruce C. Kline, Philip R. Greipp, Michael R. Loken, S-phase detection with an antibody to bromodeoxyuridine. Role of DNase pretreatment Journal of Immunological Methods. ,vol. 93, pp. 97- 101 ,(1986) , 10.1016/0022-1759(86)90438-2
Bo Dupont, Michael F. Seldin, Robert R. Rich, Human Immune Responses to Hapten-Conjugated Cells: II. The Roles of Autologous and Allogeneic Histocompatibility Determinants in Proliferative Responses in Vitro Journal of Immunology. ,vol. 122, pp. 1828- 1833 ,(1979)
J. E. Cleaver, Thymidine metabolism and cell kinetics ,(1967)
Onur Boyman, Bystander activation of CD4+ T cells. European Journal of Immunology. ,vol. 40, pp. 936- 939 ,(2010) , 10.1002/EJI.201040466
R E Tigelaar, P R Bergstresser, S Sullivan, J W Streilein, Induction and regulation of contact hypersensitivity by resident, bone marrow-derived, dendritic epidermal cells: Langerhans cells and Thy-1+ epidermal cells. Journal of Immunology. ,vol. 137, pp. 2460- 2467 ,(1986)
Sanja Gregurić, Jasna Lipozenčić, Ružica Jurakić Tončić, Ivana Martinac, Immunology of allergic contact dermatitis Acta Dermatovenerologica Croatica. ,vol. 19, pp. 51- 68 ,(2011)
A von Bonin, H U Weltzien, B Ortmann, E Schiltz, H Hoschützky, S Martin, Synthetic peptides anchor T cell-specific TNP epitopes to MHC antigens. Journal of Immunology. ,vol. 148, pp. 1445- 1450 ,(1992)
A Kapp, H Renz, M Hentschel, T Werfel, Dichotomy of blood- and skin-derived IL-4-producing allergen-specific T cells and restricted V beta repertoire in nickel-mediated contact dermatitis. Journal of Immunology. ,vol. 158, pp. 2500- 2505 ,(1997)
H U Weltzien, B Ortmann, U Birsner, S Martin, U Pflugfelder, Role of hapten-anchoring peptides in defining hapten-epitopes for MHC-restricted cytotoxic T cells. Cross-reactive TNP-determinants on different peptides. Journal of Immunology. ,vol. 149, pp. 2569- 2575 ,(1992)