Identification of Tuber spp and corresponding ectomycorrhizae through molecular markers
作者： Francesco Paolocci , Paola Angelini , Ettore Cristofari , Bruno Granetti , Sergio Arcioni
关键词: Mycorrhiza 、 Botany 、 Tuber brumale 、 Ribosomal DNA 、 Specific identification 、 Ectomycorrhizae 、 Spacer DNA 、 Tuber melanosporum 、 Biology 、 Genetic marker
摘要: Analyses, based on the structure of rDNA region, were developed to examine possibility identifying and typing some most important Tuber spp at both symbiotic non-symbiotic levels. Internal transcribed spacers (ITS) amplified by polymerase chain reaction using specific primer pairs genomic DNAs, isolated from ascocarps ectomycorrhizae various rootlets uninoculated host plants. The variability in lengths ITS amplification products DNA fruit bodies allowed a primary discrimination considered. At ectomycorrhizal level same species showed pattern which was combination those typical plus Southern analyses, performed product brumale as probe, confirmed previous results : each fungus pattern, well Tuber-host plant combination. It is concluded that molecular markers region can be used differentiate method proposed could check certify mycorrhizally infected
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