Nested-PCR for Detecting Terfezia claveryi in Roots of Helianthemum Species in Field and Greenhouse Conditions
作者： S. Jamali , Z. Banihashemi
DOI:
关键词:
摘要: Roots of Helianthemum species were collected from various rangeland sites in Fars, and other provinces Iran. The partial small subunits ribosomal DNA genes amplified with the genomic extracted their roots by nested polymerase chain reaction (PCR) using universal fungal primer pair ITS1/ITS4 specific FTC/RTC, which was designed based on internal transcribed spacer 1, 2 5.8S gene rDNA sequences Terfezia claveryi. nested-PCR sensitive enough to re-amplify direct-PCR product, resulting a fragment 500 bp. efficacy showed that it could product detect 2fg DNA. Restriction length polymorphism (RFLP) analyzed two restriction enzymes Hinf I Alu I. Nucleotides sequence analysis revealed infected close those T. claveryi . With PCR method, H. lipii salicifolium confirmed as host plants greenhouse inoculated also rangelands different areas Fars
cabdirect.org
modares.ac.ir参考文章(35)
L. J. L. D. van Griensven, Science and cultivation of edible fungi. Science and cultivation of edible fungi.. ,(2000)
A. Morte, C. Lovisolo, A. Schubert, Effect of drought stress on growth and water relations of the mycorrhizal association Helianthemum almeriense-Terfezia claveryi. Mycorrhiza. ,vol. 10, pp. 115- 119 ,(2000) , 10.1007/S005720000066
Francesco Paolocci, Paola Angelini, Ettore Cristofari, Bruno Granetti, Sergio Arcioni, Identification of Tuber spp and corresponding ectomycorrhizae through molecular markers Journal of the Science of Food and Agriculture. ,vol. 69, pp. 511- 517 ,(1995) , 10.1002/JSFA.2740690416
Francesco Paolocci, Andrea Rubini, Bruno Granetti, Sergio Arcioni, Typing Tuber melanosporum and Chinese black truffle species by molecular markers Fems Microbiology Letters. ,vol. 153, pp. 255- 260 ,(1997) , 10.1111/J.1574-6968.1997.TB12582.X
Delphine Gandeboeuf, Chantal Dupré, Gérard Chevalier, Paul Nicolas, Patricia Roeckel-Drevet, Typing Tuber ectomycorrhizae by polymerase chain amplification of the internal transcribed spacer of rDNA and the sequence characterized amplified region markers Canadian Journal of Microbiology. ,vol. 43, pp. 723- 728 ,(1997) , 10.1139/M97-104
A. Guti�rrez, A. Morte, M. Honrubia, Morphological characterization of the mycorrhiza formed by Helianthemum almeriense Pau with Terfezia claveryi Chatin and Picoa lefebvrei (Pat.) Maire. Mycorrhiza. ,vol. 13, pp. 299- 307 ,(2003) , 10.1007/S00572-003-0236-7
Antonella Amicucci, Chiara Guidi, Alessandra Zambonelli, Lucia Potenza, Vilberto Stocchi, Molecular approaches for the detection of truffle species in processed food products Journal of the Science of Food and Agriculture. ,vol. 82, pp. 1391- 1397 ,(2002) , 10.1002/JSFA.1196
M. T. Andersen, R. E. Beever, A. C. Gilman, L. W. Liefting, E. Balmori, D. L. Beck, P. W. Sutherland, G. T. Bryan, R. C. Gardner, R. L. S. Forster, Detection of phormium yellow leaf phytoplasma in New Zealand flax (Phormium tenax) using nested PCRs Plant Pathology. ,vol. 47, pp. 188- 196 ,(1998) , 10.1046/J.1365-3059.1998.00209.X
Gábor M. Kovács, Erzsébet Jakucs, István Bagi, Identification of host plants and description of sclerotia of the truffle Mattirolomyces terfezioides Mycological Progress. ,vol. 6, pp. 19- 26 ,(2007) , 10.1007/S11557-006-0520-Y
Nathalie Séjalon-Delmas, Christophe Roux, Monique Martins, Michel Kulifaj, Guillaume Bécard, Robert Dargent, Molecular tools for the identification of Tuber melanosporum in agroindustry. Journal of Agricultural and Food Chemistry. ,vol. 48, pp. 2608- 2613 ,(2000) , 10.1021/JF9910382