Integration of the delta-endotoxin gene of Bacillus thuringiensis into the chromosome of root-colonizing strains of pseudomonads using Tn5
作者： Mark G. Obukowicz , Frederick J. Perlak , Kuniko Kusano-Kretzmer , Ernest J. Mayer , L.S. Watrud
DOI: 10.1016/0378-1119(86)90031-4
关键词: Gene 、 Agrobacterium radiobacter 、 Genetic transfer 、 Pseudomonas fluorescens 、 Delta endotoxin 、 Biology 、 Genetics 、 Plasmid 、 Microbiology 、 Transposable element 、 Bacillus thuringiensis
摘要: The delta-endotoxin gene (tox) from Bacillus thuringiensis subsp. kurstaki HD-1 was cloned into Tn5 and the resulting Tn5-tox element transposed a vector plasmid chromosome of six corn-root-colonizing strains Pseudomonas fluorescens Agrobacterium radiobacter. Chromosomal integration tox maximized stability minimized potential for horizontal transfer to other bacterial species. Expression demonstrated by Western blot analysis toxicity against larvae tobacco hornworm (Manduca sexta). method described illustrates how given can be stably integrated diverse
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