Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane
作者： William Cloete , Stefan Hayward , Pieter Swart , Bert Klumperman
DOI: 10.3390/MOLECULES24030508
关键词: Starch 、 Catalysis 、 Maleic anhydride 、 Enzyme 、 Organic chemistry 、 Covalent bond 、 Amylase 、 Protease 、 Chemistry 、 Nanofiber
摘要: Two commercially available enzymes, Dextrozyme (α-amylase) and Esperase (protease), were covalently immobilized on non-woven electrospun poly(styrene-co-maleic anhydride) nanofiber mats with partial retention of their catalytic activity. Immobilization was achieved for the enzymes own as well in different combinations an additional enzyme, β-galactosidase, same mat. This experiment yielded a universal method immobilizing nanofibrous containing maleic anhydride (MAnh) residues polymer backbone.
mdpi.com
sci-hub.se 参考文章(31)
Ana L. Cordeiro, Carsten Werner, Enzymes for Antifouling Strategies Journal of Adhesion Science and Technology. ,vol. 25, pp. 2317- 2344 ,(2011) , 10.1163/016942411X574961
E.I. Zaharieva, G.G. Georgiev, Chr.I. Konstantinov, Coated reactive carriers based on copolymers of 1-vinyl-2-pyrrolidone and maleic anhydride for immobilization of enzymes. Biomaterials. ,vol. 17, pp. 1609- 1613 ,(1996) , 10.1016/0142-9612(95)00318-5
Andreas Liese, Lutz Hilterhaus, Evaluation of immobilized enzymes for industrial applications Chemical Society Reviews. ,vol. 42, pp. 6236- 6249 ,(2013) , 10.1039/C3CS35511J
Ana L. Cordeiro, Catharina Hippius, Carsten Werner, Immobilized enzymes affect biofilm formation. Biotechnology Letters. ,vol. 33, pp. 1897- 1904 ,(2011) , 10.1007/S10529-011-0643-3
Suzanne Hilton, William D. McCubbin, Cyril M. Kay, J. Thomas Buckley, Purification and spectral study of a microbial fatty acyltransferase: activation by limited proteolysis. Biochemistry. ,vol. 29, pp. 9072- 9078 ,(1990) , 10.1021/BI00490A026
Peng Ye, Zhi-Kang Xu, Jian Wu, Christophe Innocent, Patrick Seta, Nanofibrous Membranes Containing Reactive Groups: Electrospinning from Poly(acrylonitrile-co-maleic acid) for Lipase Immobilization Macromolecules. ,vol. 39, pp. 1041- 1045 ,(2006) , 10.1021/MA0517998
Tilo Pompe, Stefan Zschoche, Nicole Herold, Katrin Salchert, Marie-Francoise Gouzy, Claudia Sperling, Carsten Werner, Maleic anhydride copolymers--a versatile platform for molecular biosurface engineering. Biomacromolecules. ,vol. 4, pp. 1072- 1079 ,(2003) , 10.1021/BM034071C
Zhen-Gang Wang, Ling-Shu Wan, Zhen-Mei Liu, Xiao-Jun Huang, Zhi-Kang Xu, Enzyme immobilization on electrospun polymer nanofibers: An overview Journal of Molecular Catalysis B-enzymatic. ,vol. 56, pp. 189- 195 ,(2009) , 10.1016/J.MOLCATB.2008.05.005
Neelam Gurung, Sumanta Ray, Sutapa Bose, Vivek Rai, A Broader View: Microbial Enzymes and Their Relevance in Industries, Medicine, and Beyond BioMed Research International. ,vol. 2013, pp. 329121- 329121 ,(2013) , 10.1155/2013/329121
Kyunghwan Yoon, Benjamin S. Hsiao, Benjamin Chu, High flux nanofiltration membranes based on interfacially polymerized polyamide barrier layer on polyacrylonitrile nanofibrous scaffolds Journal of Membrane Science. ,vol. 326, pp. 484- 492 ,(2009) , 10.1016/J.MEMSCI.2008.10.023