作者: E.I. Zaharieva , G.G. Georgiev , Chr.I. Konstantinov
DOI: 10.1016/0142-9612(95)00318-5
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摘要: Surface-activated silicas with methacrylate double bonds were introduced into a reaction medium where copolymerization of 1-vinyl-2-pyrrolidone (VP) and maleic anhydride (MA) took place. The led to the formation non-alternating copolymers VP MA. Each macromolecule this copolymer consisted alternately arranged regulated long sequences units single possibility for covalent bonding β-galactosidase MA at different lengths was studied. content coupled enzyme, as well its activity, pH dependence stability determined.