Immobilization of glucose oxidase by acrylonitrile copolymer coated silica supports

摘要: New polymer/silica gel hybrid supports were prepared by coating high surface area of silica with eight different copolymers acrylonitrile: acrylonitrile-vinyl pyridine, acrylonitrile-vinylimidazole, acrylonitrile-N,N-dirnethylaminoethylmethacrylate, acrylonitrile-acrylic acid, acrylonitrile-2,6-dichlorphenilmaleimide, acrylonitrile-maleic anhydride, acrylonitrile-hydroxyethylmethacrylate and acrylonitrile-methylmethacrylate Na-vinylsulfonate. Glucose oxidase was covalently immobilized on six types acrylonitrile copolymer/silica supports. Glutaraldehyde used as coupling agent between the support treated hexamethylenediamine (spacer) enzyme in case S A N - M , H E . c ( D ) SPAN- The carboxylic groups activated N,N'-dicyclohexylcarbodiimide C I for covalent immobilization glucose oxidase. Direct achieved P via reactive Cl-ions e-amino group lysine residue enzyme. V adsorption ionic bonding. greatest amount bound protein relative activity presented among SPAN bonding is also since no conformation changes molecules could be noticed. system showed highest thermal stability at 60 °C (78% residual fifth hour), followed GOD (72 70%). most stable 50 days storage time (80% activity) (73%).