Expression of the Proopiomelanocortin Gene in Human Medullary Thyroid Carcinoma
作者： P.H. STEENBERGH , J.W.M. HÖPPENER , J. ZANDBERG , B. A. ROOS , H. S. JANSZ
关键词: Messenger RNA 、 Pituitary gland 、 Thyroid carcinoma 、 RNA 、 Thyroid 、 Molecular biology 、 Proopiomelanocortin 、 Internal medicine 、 Endocrinology 、 Calcitonin 、 Biology 、 Medullary carcinoma
摘要: mRNA isolated from medullary carcinoma of the thyroid (MTC) in six patients with inherited multiple endocrine neoplasia syndrome type 2 and cervical metastases two sporadic MTC was screened for presence calcitonin proopiomelanocortin (POMC) related sequences by blot hybridization analysis. All tumors were found to contain hybridizing a calcitonin–specific probe. However, POMC-related detected only RNA preparations purified MTC. Compared POMC occurring human pituitary gland, size species POMC-specific probe larger ofboth investigated. cDNA complementary one cloned into pBR 322. From this library plasmids containing inserts which completely homologous sequence 190 nucleotides 3′-end mRNA. In conclusion, 1) nucleotide sequ...
oup.com
endocrine.org
endojournals.org参考文章(14)
F Sundler, O Ljungberg, L Björklund, J Alumets, R Håkanson, Somatostatin-immunoreactive cells in medullary carcinoma of the thyroid. American Journal of Pathology. ,vol. 88, pp. 381- 386 ,(1977)
ALTON L. STEINER, A DAVID GOODMAN, SAMUEL R. POWERS, Study of a kindred with pheochromocytoma, medullary thyroid carcinoma, hyperparathyroidism and Cushing's disease: multiple endocrine neoplasia, type 2. Medicine. ,vol. 47, pp. 371- 409 ,(1968) , 10.1097/00005792-196809000-00001
C. DeBold, M. Schworer, T. Connor, R. Bird, D. Orth, Ectopic pro-opiolipomelanocortin: sequence of cDNA coding for beta-melanocyte-stimulating hormone and beta-endorphin. Science. ,vol. 220, pp. 721- 723 ,(1983) , 10.1126/SCIENCE.6301015
A. M. Maxam, W. Gilbert, A new method for sequencing DNA Proceedings of the National Academy of Sciences of the United States of America. ,vol. 74, pp. 560- 564 ,(1977) , 10.1073/PNAS.74.2.560
Tamotsu Shibasaki, Leonard Deftos, Roger Guillemin, Immunoreactive-β-endorphin, -adrenocorticotropin and -calcitonin in extracts of anaplastic or differentiated (rat) medullary thyroid carcinoma Biochemical and Biophysical Research Communications. ,vol. 90, pp. 1266- 1273 ,(1979) , 10.1016/0006-291X(79)91173-2
LEONARD J. DEFTOS, HENRY G. BONE, JACQUELINE G. PARTHEMORE, DOUGLAS W. BURTON, Immunohistological Studies of Medullary Thyroid Carcinoma and C Cell Hyperplasia The Journal of Clinical Endocrinology and Metabolism. ,vol. 51, pp. 857- 862 ,(1980) , 10.1210/JCEM-51-4-857
DAVID GOLTZMAN, SHAO-NAN HUANG, CHRISTOPHER BROWNE, SAMUEL SOLOMON, Adrenocorticotropin and calcitonin in medullary thyroid carcinoma: frequency of occurrence and localization in the same cell type by immunocytochemistry. The Journal of Clinical Endocrinology and Metabolism. ,vol. 49, pp. 364- 369 ,(1979) , 10.1210/JCEM-49-3-364
W. L. Miller, L. K. Johnson, J. D. Baxter, J. L. Roberts, Processing of the precursor to corticotropin and beta-lipotropin in humans. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 5211- 5215 ,(1980) , 10.1073/PNAS.77.9.5211
Toshihiko Tsukada, Yoshikatsu Nakai, Hisato Jigami, Hiroo Imura, Shunzo Taii, Shigetada Nakanishi, Shosaku Numa, Identification of the mRNA coding for the ACTH-β-lipotropin precursor in a human ectopic ACTH-producing tumor Biochemical and Biophysical Research Communications. ,vol. 98, pp. 535- 540 ,(1981) , 10.1016/0006-291X(81)90873-1
Roger K. Craig, Len Hall, Mark R. Edbrooke, Janette Allison, Iain MacIntyre, Partial nucleotide sequence of human calcitonin precursor mRNA identifies flanking cryptic peptides. Nature. ,vol. 295, pp. 345- 347 ,(1982) , 10.1038/295345A0