Ethanol improves adenovirus-mediated gene transfer and expression to the bladder epithelium of rodents.

摘要: Abstract Objectives. Replication deficient adenoviral vectors (rAds) are used as gene delivery systems that can efficiently transduce a variety of tissues and may be appropriate to develop therapeutics for many urologic applications. However, the bladder epithelium has been shown highly resistant transgene expression after intracystic administration. A potential explanation this low transfer efficiency protective structure urothelium. Since barrier disrupted by organic solvents, we assessed whether ethanol co-administration enhance adenovirus-mediated expression. Methods. Normal tumor-bearing rats received single administration rAd encoding β-galactosidase (rAd-βgal) or p53 (rAd-p53). was administered in saline solution solutions with increasing concentrations ethanol. Transgene evaluated tissues. Results. dramatic increase urothelial achieved rAd-βgal 22% solution. enhanced normal urothelium superficial tumors. similarly enhanced. Conclusions. Co-administration local rAd-mediated neoplastic rodents. Improvement is progress toward enable therapy cancer other diseases.