Identification of Pretreatment Agents to Enhance Adenovirus Infection of Bladder Epithelium

摘要: Abstract Adenovirus has been used widely as a gene transfer vector in the laboratory and clinic for purpose of therapy. Conditionally replication-competent oncolytic adenoviruses are capable multiplying up to thousand old target cells, property that might prove be tremendous potential area cancer Intravesicular therapy refractory superficial bladder employing an adenovirus would allow local administration efficient delivery virus tumor. The glycosaminoglycan layer on surface urothelium acts nonspecific antiadherence barrier may significant roadblock infection by adenoviruses. Several laboratories have investigated utility pretreatment with chemical agents enhance but limited success. A class compounds identified is effective urothelium, permitting adenoviral infection. In murine model, 0.1% dodecyl-β- d -maltoside (DDM) or sodium dodecyl sulfate (SDS) 5 min resulted >90% transduction urothelial within 15 after exposure replication-defective compared ≤5% untreated bladders. DDM could coformulated adenovirus, complete was achieved following retention admixture 45 min. similar enhancement SDS observed rat model. use these facilitate development adenovirus-based cancer.