Anti-proliferative effect of Kv1.3 blockers in A549 human lung adenocarcinoma in vitro and in vivo.
作者： Soo Hwa Jang , Seon Young Choi , Pan Dong Ryu , So Yeong Lee , None
DOI: 10.1016/J.EJPHAR.2010.10.066
关键词: Adenocarcinoma 、 Cancer research 、 Cell culture 、 Margatoxin 、 Cell cycle 、 Pathology 、 Small hairpin RNA 、 Cancer cell 、 Biology 、 Cell growth 、 A549 cell
摘要: Voltage-gated potassium (Kv) channels are widely expressed in the plasma membranes of numerous cells and contribute to a variety cellular functions both excitable neuronal non-excitable epithelial cells. Recently, it has been demonstrated that Kv associated with proliferation several types cancer In present study, we investigated effects suppression Kv1.3 expression on cell cycle progression human lung adenocarcinoma, A549 Treatment margatoxin (MgTX), selective blocker or short hairpin RNA (shRNA) against Kv1.3, significantly blocked cells' proliferation. addition, inhibition increased level p21(Waf1/Cip1) decreased Cdk4 cyclin D3. We also applied MgTX into xenograft model using nude mice, caused reduction tumor volume when was injected tissues. These results suggest may serve as novel therapeutic target for adenocarcinoma therapy.
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