Differentiation dependency of eicosanoid enzyme expression in human tracheobronchial cells
作者： E Hill
DOI: 10.1016/S0378-4274(98)00078-2
关键词: Eicosanoid 、 Cellular differentiation 、 Prostaglandin 、 Epithelium 、 Biology 、 Cell culture 、 Molecular biology 、 Internal medicine 、 Retinoic acid 、 Endocrinology 、 Eicosanoid metabolism 、 Proinflammatory cytokine
摘要: Abstract The expression of 15-lipoxygenase (15-LO) and prostaglandin H synthase-2 (PGHS-2) was studied in retinoic acid (RA) sufficient RA-deficient normal human tracheobronchial epithelial (NHTBE) cell cultures. In the absence RA, which cultures became squamous metaplastic, neither 15-LO nor PGHS-2 were expressed. RA-sufficient cultures, differentiated into a mucociliary epithelium, both enzymes expressed: during early phases late stages differentiation. Depending on stage differentiation, produced PGE2 presence exogenous linoleic (LA) 13-HODE. Experiments are underway to examine effects inflammatory cytokines eicosanoid metabolism role these metabolites play airway diseases.
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sci-hub.se 参考文章(17)
Kevin P. Keenan, John W. Combs, Elizabeth M. McDowell, Regeneration of hamster tracheal epithelium after mechanical injury Virchows Archiv B Cell Pathology Including Molecular Pathology. ,vol. 41, pp. 193- 214 ,(1982) , 10.1007/BF02890281
K. Asano, C. M. Lilly, J. M. Drazen, Prostaglandin G/H synthase-2 is the constitutive and dominant isoform in cultured human lung epithelial cells American Journal of Physiology-lung Cellular and Molecular Physiology. ,vol. 271, ,(1996) , 10.1152/AJPLUNG.1996.271.1.L126
Hartmut Kühn, Bernd-Joachim Thiele, Arachidonate 15-hpoxygenase Journal of Lipid Mediators and Cell Signalling. ,vol. 12, pp. 157- 170 ,(1995) , 10.1016/0929-7855(95)00016-J
Hartmut Kühn, Jim Barnett, Dorit Grunberger, Preston Baecker, Joan Chow, Binh Nguyen, Hela Bursztyn-Pettegrew, Hardy Chan, Elliott Sigal, Overexpression, purification and characterization of human recombinant 15-lipoxygenase Biochimica et Biophysica Acta. ,vol. 1169, pp. 80- 89 ,(1993) , 10.1016/0005-2760(93)90085-N
J H Yoon, T Gray, K Guzman, J S Koo, P Nettesheim, Regulation of the secretory phenotype of human airway epithelium by retinoic acid, triiodothyronine, and extracellular matrix. American Journal of Respiratory Cell and Molecular Biology. ,vol. 16, pp. 724- 731 ,(1997) , 10.1165/AJRCMB.16.6.9191474
E. M. Hill, T. Eling, P. Nettesheim, Changes in Expression of 15-Lipoxygenase and Prostaglandin-H Synthase during Differentiation of Human Tracheobronchial Epithelial Cells American Journal of Respiratory Cell and Molecular Biology. ,vol. 18, pp. 662- 669 ,(1998) , 10.1165/AJRCMB.18.5.2985
P Nettesheim, Tumor necrosis factor a stimulates arachidonic acid metabolisms and mucus production in rat tracheal epithelial cell cultures Toxicology Letters. ,vol. 88, pp. 35- 37 ,(1996) , 10.1016/0378-4274(96)03715-0
Zvi Marom, James H. Shelhamer, Michael Kaliner, Effects of Arachidonic Acid, Monohydroxyeicosatetraenoic Acid and Prostaglandins on the Release of Mucous Glycoproteins from Human Airways In Vitro Journal of Clinical Investigation. ,vol. 67, pp. 1695- 1702 ,(1981) , 10.1172/JCI110207
Gadiparthi N. Rao, Wayne C. Glasgow, Thomas E. Eling, Marschall S. Runge, Role of Hydroperoxyeicosatetraenoic Acids in Oxidative Stress-induced Activating Protein 1 (AP-1) Activity Journal of Biological Chemistry. ,vol. 271, pp. 27760- 27764 ,(1996) , 10.1074/JBC.271.44.27760
Z Marom, J H Shelhamer, F Sun, M Kaliner, Human airway monohydroxyeicosatetraenoic acid generation and mucus release. Journal of Clinical Investigation. ,vol. 72, pp. 122- 127 ,(1983) , 10.1172/JCI110949