Human airway construct model is suitable for studying transcriptome changes associated with indoor air particulate matter toxicity

摘要: In vitro models mimicking the human respiratory system are essential when investigating toxicological effects of inhaled indoor air particulate matter (PM). We present a pulmonary cell culture model for studying PM toxicity. exposed normal bronchial epithelial cells, grown on semi-permeable membranes, to four doses in air-liquid interface. analyzed chemokine interleukin-8 concentration from medium, protein apical wash, measured tissue electrical resistance, and imaged airway constructs using light transmission electron microscopy. sequenced RNA targeted toxicology panel 386 genes associated with responses. was collected non-complaint residential environment over 1 week. Sample collection concomitant monitoring size-segregated counts determination microbial levels diversity. exposure not acutely toxic we observed up-regulation 34 down-regulation 17 compared blank sampler control exposure. The five most up-regulated were related immunotoxicity. Despite indications incomplete differentiation, this enabled comparison transcriptome