Bifunctional Tm3+,Yb3+:GdVO4@SiO2 Core-Shell Nanoparticles in HeLa Cells: Upconversion Luminescence Nanothermometry in the First Biological Window and Biolabelling in the Visible.

摘要: The bifunctional possibilities of Tm,Yb:GdVO4@SiO2 core-shell nanoparticles for temperature sensing by using the near-infrared (NIR)-excited upconversion emissions in first biological window, and biolabeling through visible they generate, were investigated. two emission lines located at 700 800 nm, that arise from thermally coupled 3F2,3 3H4 energy levels Tm3+, used to develop a luminescent thermometer, operating Fluorescence Intensity Ratio (FIR) technique, with very high thermal relative sensitivity. Moreover, since inert shell surrounding active core allows dispersal water compatible fluids, we investigated penetration depth can be realized tissues their NIR range, achieving value 0.8 mm when excited powers 50 mW. After internalization HeLa cells, low toxicity was observed potentiality biolabelling range demonstrated, which facilitated identification location inside determination.