Establishment of ten strains of genetically engineered Salmonella typhimurium TA1538 each co-expressing a form of human cytochrome P450 with NADPH-cytochrome P450 reductase sensitive to various promutagens.
作者： Yoshiyuki Yamazaki , Ken-ichi Fujita , Kazuo Nakayama , Akihiro Suzuki , Katsunori Nakamura
DOI: 10.1016/J.MRGENTOX.2004.06.003
关键词: CYP1A2 、 Biochemistry 、 Enzyme 、 Reductase 、 2-Amino-1-methyl-6-phenylimidazo(4,5-b)pyridine 、 Benzo(a)pyrene 、 Enterobacteriaceae 、 Cytochrome P450 、 2-Acetylaminofluorene 、 Chemistry
摘要: We newly developed 10 Salmonela typhimurium TA1538 strains each co-expressing a form of human cytochrome P450s (P450 or CYP) together with NADPH-cytochrome P450 reductase (CPR) for highly sensitive detection mutagenic activation mycotoxins, polycyclic aromatic hydrocarbons, heterocyclic amines, and amines at low substrate concentrations. Each (CYP1A1, CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A4 CYP3A5) expressed in the cells efficiently catalyzed oxidation representative substrate. Aflatoxin B1 was mutagenically activated effectively by CYP1A1, weakly CYP2A6 CYP2C8 S. TA1538. CYP1A1 CYP1A2 were responsible 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) 2-acetylaminofluorene. Benzo[a]pyrene also These results suggest that are applicable detecting promutagens which is not detectable N-nitrosamine-sensitive YG7108 expressing P450s.
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