Haemodialysis as a model for studying endogenous plasma DNA: oligonucleosome-like structure and clearance.

摘要: The rate of clearance extracellular plasma DNA in man has important implications for pathogenetic mechanisms systemic lupus erythematosus (SLE), as well certain other clinical states. Present knowledge this parameter is derived exclusively from studies injected, naked animals. Recent information indicates that the physiologic form SLE oligonucleosome-like molecules rather than and consists multimeric complexes bound to histone, probably arising an apoptotic process. In order study at which these are removed do so experimental animals, we exploited observation during haemodialysis large amounts released, apparently within dialysis coil, into patient's plasma. Since release appears cease promptly with termination procedure, it offered potential estimating removal such human Moreover, if DNA, postulated, were shown possess structure resembling found endogenously SLE, relevance disease state would be further enhanced. present results support conclusion released possesses structure. half-life was not exceed 4 min. highly dynamic thus implied endogenous dependent on dsDNA SLE. individuals undergoing chronic haemodialysis, who thereby exposed a very cumulative amount might serve models studying its long-term sequelae.