The ABRF Proteomics Research Group Studies: Educational exercises for qualitative and quantitative proteomic analyses

摘要: Resource (core) facilities have played an ever-increasing role in furnishing the scientific community with specialized instrumentation and expertise for proteomics experiments a cost-effective manner. The Proteomics Research Group (PRG) of Association Biomolecular Facilities (ABRF) has sponsored number research studies designed to enable participants try new techniques assess their capabilities relative other laboratories analyzing same samples. Presented here are results from three PRG representing different samples that typically analyzed core facility, ranging simple protein identification targeted analyses, include intentional challenges reflect realistic studies. PRG2008 study compares strategies qualitative characterization proteins, particularly utility complementary methods characterizing truncated forms. use approaches determining quantitative differences several target proteins human plasma was focus PRG2009 study. PRG2010 explored specific constituents while identifying unforeseen problems could account unanticipated associated samples, included (15) N-labeled as additional challenge. These provide valuable educational resource facilities, well mechanism establishing good laboratory practices.