Mass spectrometry‐based targeted quantitative proteomics: Achieving sensitive and reproducible detection of proteins

摘要: Traditional shotgun proteomics used to detect a mixture of hundreds thousands proteins through mass spectrometric analysis, has been the standard approach in research profile protein content biological sample which could lead discovery new (and all) candidates with diagnostic, prognostic, and therapeutic values. In practice, this requires significant resources time, does not necessarily represent goal researcher who would rather study subset such discovered (including their variations or posttranslational modifications) under different conditions. context, targeted is playing an increasingly important role accurate measurement targets samples hope elucidating molecular mechanism cellular function via understanding intricate networks pathways. One (targeted) approach, selected reaction monitoring (or multiple monitoring) spectrometry (MRM-MS), offers capability measuring higher sensitivity throughput than proteomics. Developing validating MRM-MS-based assays, however, extensive iterative process, requiring coordinated collaborative effort by scientific community sharing publicly accessible data datasets, bioinformatic tools, operating procedures, well characterized reagents.