Hyperplasia of Pericytes Is One of the Main Characteristics of Microvascular Architecture in Malignant Glioma

摘要: Objectives To investigate the role of pericytes in constructing malformed microvessels (MVs) and participating microvascular architecture heterogeneity glioma. Methods Forty human glioma tissue samples (WHO grade II-IV) were included present study. Observation blood vessel patterns, quantitative analysis endothelial cells (ECs)- pericyte-labeled MVs comparison between malignant grades based on single- or double-immunohistochemical staining. The MV number density (MVND), pericyte (MPND), area (MPAD) calculated. expression PDGFβ was also scored after immunostaining. Results In II glioma, most tumor thin-wall CD34+ vessels with near normal morphology. In addition to MVs, more thick-wall found III which often showed α-SMA positive. Most IV form plexus, curled cell cords glomeruloid proliferation while α-SMA+ main components. usually disordered arrangement, loose connection active as shown by Ki67 coexpression. With increase grades, MVND, MVND MPND significantly augmented although but not MPAD statistically insignificant IV. It interesting that some vessel-like structures only consist cells, assuming guiding angiogenesis. level upregulated directly correlated different grades. Conclusion Hyperplasia one significant characteristics locally proliferated constituent high glioma. pathological could be used indexes