Intracranial injection of human meningioma cells in athymic mice: an orthotopic model for meningioma growth.

摘要: Object. Although human meningioma cells have been heterotopically implanted in nude mice, introducing these into intracranial locations seems more likely to reproduce normal patterns of tumor growth. To provide an orthotopic xenograft model meningioma, the authors a controlled quantity at subdural and intracerebral sites athymic mice. Methods. Malignant (one tumor), atypical (two tumors), or benign (three tumors) meningiomas were placed primary cell cultures. Cells (106/10 µl) from cultures immortalized malignant line, IOMM-Lee, injected with stereotactic guidance frontal white matter space Survival curves plotted for mice receiving each histological type according injection site. Other killed intervals their heads sectioned whole. Hematoxylin eosin staining sections revealed extent Conclusions. The me...