Proteomic profile of platelets during reconstitution of platelet counts after apheresis
作者： Thomas Thiele , Johannes Braune , Vishnu Dhople , Elke Hammer , Christian Scharf
关键词: Proteome 、 Moesin 、 Proteomic Profile 、 Pharmacology 、 Protein subunit 、 Platelet 、 GTPase-activating protein 、 Proteomics 、 Chemistry 、 40S Ribosomal Protein SA
摘要: Purpose Circulating platelets consist of subpopulations different age. We designed an approach to remove from circulation using platelet apheresis. aimed detect changes in the proteome related increased turnover after apheresis map candidate proteins, which may serve as markers young platelets. Experimental design A healthy donor underwent three procedures on consecutive days. Blood was drawn at day 1 (baseline) and days 3, 5, 7, 9, 11 for analysis LC-ESI-MS/MS 2D-DIGE. Results Among 1017 proteins identified by LC-ESI-MS/MS, 54 changed quantity throughout course study. Potential were 40S ribosomal protein SA, COP9 signalosome complex subunit involved clathrin-mediated endocytosis signaling. 1036 spots observed 2D-DIGE, 45 displayed fluorescence intensity. Identified contained IQ motif containing GTPase activating 2, talin, moesin, myosin regulatory light chain coronin-1C. Conclusions clinical relevance provide proof principle that a combination proteomic approaches enables identification are de novo synthesis.
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