Pulsed electric field exposure of insulin induces anti-proliferative effects on human hepatocytes.

摘要: Thepurposeofthisstudywastoexaminetheeffectsandthemechanismofapulsedelectricfield(PEF) on insulin and its subsequent mediation of proliferative changes in human hepatocytes vitro. The PEF, the electric field intensity coupled into culture medium, was about 0.7 V/m with a repeating frequency 50 Hz. Insulin solution exposed to PEF for 20 min added culturemediumofhumanhepatocytes.Combiningfluorescencespectroscopy,immunocytochemistry, microarrays, RT-PCR MTT, several important events signaling pathways were investigated, including ligand-receptor binding capacity, intracellular tyrosine phosphorylation level, genetranscription, andcell proliferation.PEFproduceda conformational changeofinsulinmolecule. capacity receptors reduced 87% control level after treatment, average decreased by 11%. expression 55 12000 genes examined modified, an increase phosphatase small GTP-binding protein. Based these results, mechanism isproposedtoexplainthebio-effectsofPEFonhepaticcellproliferationthroughtheinsulinsignaling pathway. Bioelectromagnetics 26, 2005. � 2005 Wiley-Liss, Inc.