Regulation of epidermal growth factor receptor in human colon cancer cell lines by interferon  

摘要: Background and aim: The biology of growth factor receptor expression has implications for specific cancer therapy. In this study, we examined: (a) regulation epidermal (EGFR) in a panel 10 human colon cell lines using interferon α (IFN-α); (b) ability IFN-α to inhibit proliferation; (c) sensitivity pretreated cells EGF. Methods: Cell proliferation was measured both by crystal violet colorimetric clonogenic assays. surface, intracellular, and/or total protein EGFR assessed indirect immunofluorescence flow cytometry fluorescein isothiocyanate (FITC)-EGF binding internalisation cytometric assay. Results: treatment upregulated surface seven within 16 hours, reaching peak 48–96 hours; accompanied transient elevation intracellular marked inhibition. treated were still sensitive EGF proliferative stimulation. Conclusions: Our results indicate that cytostatic concentrations can enhance proportion cells. antiproliferative action could not block the signal transduction EGF-EGFR pathway. This may have clinical improving based on targeting EGFR.