Efficient gene delivery by EGF-lipoplexes in vitro and in vivo
作者： María Buñuales , Nejat Düzgüneş , Sara Zalba , Maria J Garrido , Conchita Tros de ILarduya
DOI: 10.2217/NNM.10.100
关键词:
摘要: Aims: In this work, we have evaluated the ability of targeted lipoplexes to enhance transgene expression in EGF receptor (EGFR) overexpressing tumor cells by using lipoplexes. Materials & methods: We prepared DOTAP/cholesterol liposomes modified with at 0.5/1, 1/1, 2/1 and 5/1 lipid/DNA (+/-) charge ratio sequentially mixing ligand adding reporter or therapeutic plasmid gene, pCMVLuc (pVR1216) pCMVIL12, respectively. HepG2, DHDK12proB SW620 were used for vitro experiments, which performed presence 60% serum. Results: The characterization EGF-lipoplexes indicated a size close 300 nm variable net surface as function amount associated cationic liposomes. EGF-lipoplexes, showed an increased transfection activity, positively charged, noncytotoxic highly effective protecting DNA from DNase I attack. Transfection activity resulted enhancement luciferase IL...
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