Reciprocal changes in p27(Kip1) and p21(Cip1) in growth inhibition mediated by blockade or overstimulation of epidermal growth factor receptors.
作者： Zhen Fan , Jia Ling Chou , Yang Lu , Barbara Y. Shang , John Mendelsohn
DOI:
关键词: Epidermoid carcinoma 、 Receptor 、 Growth inhibition 、 Cell growth 、 Internal medicine 、 Biology 、 Epidermal growth factor 、 Cell biology 、 Endocrinology 、 Stimulation 、 Mechanism of action 、 A431 cells
摘要: Many human epithelial tumors express high levels of epidermal growth factor (EGF) receptors. A human-mouse chimeric version anti-EGF receptor monoclonal antibody (mAb) C225, which blocks activation and produces inhibition cell proliferation, is currently being investigated in clinical trials. When cells bear numbers EGF receptors, either complete blockade receptors with mAb 225 or full results proliferation. In the present study, we have explored molecular mechanisms explaining how a inhibitor, 225, activator, EGF, can both produce A431 squamous carcinoma cells. We reported previously that by associated up-regulation p21(Cip1). now demonstrate 255-mediated p27(Kip1), binds to inactivates cyclin-dependent kinase-2 activity cycle arrest G1. Furthermore, be overcome titrating cultures increasing concentrations accompanied concurrent fall level p27(Kip1). At properly titrated inhibitory activities are counterbalanced abolished. reach enough compete near-saturating activation, p27(Kip1) falls below basal levels; however, concomitant marked rise p21(Cip1) inhibition. Our data suggest although induced act independently, they play reciprocal roles mediating saturating EGF.
aacrjournals.org参考文章(24)
Tony DeBlasio, Mark Rubin, Zhen Fan, Andrew Koff, Tim Soos, John Mendelsohn, Xipu Wu, Involvement of p27KIP1 in G1 arrest mediated by an anti-epidermal growth factor receptor monoclonal antibody. Oncogene. ,vol. 12, pp. 1397- 1403 ,(1996)
Hideo Masui, Ian Altas, John Mendelsohn, Zhen Fan, Blockade of epidermal growth factor receptor function by bivalent and monovalent fragments of 225 anti-epidermal growth factor receptor monoclonal antibodies Cancer Research. ,vol. 53, pp. 4322- 4328 ,(1993)
Z Fan, Y Lu, X Wu, J Mendelsohn, Antibody-induced epidermal growth factor receptor dimerization mediates inhibition of autocrine proliferation of A431 squamous carcinoma cells. Journal of Biological Chemistry. ,vol. 269, pp. 27595- 27602 ,(1994) , 10.1016/S0021-9258(18)47026-3
Zhen Fan, Hideo Masui, John Mendelsohn, Jose Baselga, Antitumor Effect of Anti-Epidermal Growth Factor Receptor Monoclonal Antibodies plus cis-Diamminedichloroplatinum on Well Established A431 Cell Xenografts Cancer Research. ,vol. 53, pp. 4637- 4642 ,(1993)
G N Gill, T Kawamoto, C Cochet, A Le, J D Sato, H Masui, C McLeod, J Mendelsohn, Monoclonal anti-epidermal growth factor receptor antibodies which are inhibitors of epidermal growth factor binding and antagonists of epidermal growth factor binding and antagonists of epidermal growth factor-stimulated tyrosine protein kinase activity. Journal of Biological Chemistry. ,vol. 259, pp. 7755- 7760 ,(1984) , 10.1016/S0021-9258(17)42857-2
Anthony DeBlasio, Joachim Yahalom, Mark Rubin, Huisheng Wang, Robert Wieder, Eyal Fenig, John Mendelsohn, Basic Fibroblast Growth Factor Causes Growth Arrest in MCF-7 Human Breast Cancer Cells while Inducing both Mitogenic and Inhibitory G1 Events Cancer Research. ,vol. 57, pp. 1750- 1757 ,(1997)
Frank Cuttitta, William E. Karnes, S. Vincent Wu, John H. Walsh, Jae-Gahb Park, Martin G. Martin, Helen C. Wong, Richard S. Kim, John Mendelsohn, Autonomous proliferation of colon cancer cells that coexpress transforming growth factor α and its receptor Gastroenterology. ,vol. 102, pp. 474- 485 ,(1992) , 10.5555/URI:PII:001650859290093E
M J Van de Vijver, R Kumar, J Mendelsohn, Ligand-induced activation of A431 cell epidermal growth factor receptors occurs primarily by an autocrine pathway that acts upon receptors on the surface rather than intracellularly. Journal of Biological Chemistry. ,vol. 266, pp. 7503- 7508 ,(1991) , 10.1016/S0021-9258(20)89475-7
T Kawamoto, J Mendelsohn, A Le, G H Sato, C S Lazar, G N Gill, Relation of epidermal growth factor receptor concentration to growth of human epidermoid carcinoma A431 cells. Journal of Biological Chemistry. ,vol. 259, pp. 7761- 7766 ,(1984) , 10.1016/S0021-9258(17)42858-4
Jordan U. Gutterman, David V. Goeddel, Richard D. Williams, Axel Ullrich, Rik Derynck, Wendy H. Berger, Timothy S. Bringman, Synthesis of Messenger RNAs for Transforming Growth Factors α and β and the Epidermal Growth Factor Receptor by Human Tumors Cancer Research. ,vol. 47, pp. 707- 712 ,(1987)