Monoclonal anti-epidermal growth factor receptor antibodies which are inhibitors of epidermal growth factor binding and antagonists of epidermal growth factor binding and antagonists of epidermal growth factor-stimulated tyrosine protein kinase activity.

摘要: Four mouse monoclonal antibodies specific for human epidermal growth factor (EGF) receptors have been prepared using EGF receptor protein from A431 epidermoid carcinoma cells as immunogen. We determined the effect of these on two known functions receptor: binding and tyrosine kinase. Three (225, 528, 579) are inhibitors binding, whereas fourth (455) does not compete but immunoprecipitates receptor. Inhibition is mixed competitive noncompetitive type. The three competing antagonists EGF-stimulated kinase activity assayed both in intact an exogenous peptide substrate solubilized membranes. These immunoglobulins partial agonists self-phosphorylation membranes exhibit only antagonist this reaction cells. recognize variant with same efficiency parental Such can be used to control concentration which activated by EGF.