Antileishmanial, antioxidant, and cytotoxic activities of Quercus infectoria Olivier extract.

作者: Farnaz Kheirandish , Bahram Delfan , Hossein Mahmoudvand , Nasim Moradi , Behrouz Ezatpour

DOI: 10.1016/J.BIOPHA.2016.04.040

关键词: ChemistryQuercus infectoriaTraditional medicineGallic acidFlavonoidLeishmania majorBiphenyl compoundDPPHMTT assayBiochemistryQuercetin

摘要: Currently, there is no effective vaccine available, and chemotherapy the main approach for treatment of cutaneous leishmaniasis (CL). During recent decades, studies have demonstrated that a number plant-derived compounds may act as new therapeutic tools against leishmaniasis. This study was evaluated antileishmanial, antioxidant, cytotoxic activities Quercus infectoria Olivier (oak) extract. The total amount phenolic flavonoid measured in oak High performance liquid chromatography (HPLC) analysis also performed to determine quercetin gallic acid this plant. extract (0-80g/mL) vitro promastigote intracellular amastigote forms Leishmania major (MRHO/IR/75/ER) using MTT assay macro-phage model, respectively. Then tested on CL infected male BALB/c mice with L. order evaluate antileishmanial activity topically. Moreover, cytotoxicity effects murine macrophage cells were by assay. Antioxidative determined 2,2-diphenyl-1,1-picrylhydrazyl (DPPH) scavenging test. 57.50 1.86%, 0.0064 0.22%, findings revealed significantly (P<0.05) inhibited growth rate (IC50 12.65μg/mL) amastigotes 10.31μg/mL) dose-dependent response. In vivo assay, after 4 weeks treatment, 91.6, 66.66, 50% recovery observed treated 20, 10, 5mg/kg extract, After concentration 10 20mg/kg oak, mean diameter lesions, parasite load parasites reduced. Selectivity index greater than had cells. DPPH test radical inhibition occurred at power increasing oak. To conclude, present showed potent antioxidant extract; whereas plant toxic effect mammalian

参考文章(54)
R. N. Chopra, I. C. Chopra, S. L. Nayar, Glossary of Indian Medicinal Plants ,(1956)
Mohammad Hassan Kayedi, Kiumars Khamisabadi, Nader Dehghani, Ali Akbar Haghdoost, None, Entomological evaluation of PermaNet 2.0® and K-O Tab 1-2-3® treated nets in comparison to nets conventionally treated with deltamethrin, after repeated washing. Pathogens and Global Health. ,vol. 109, pp. 196- 201 ,(2015) , 10.1179/2047773215Y.0000000010
Mohammad S. Dar, M. Ikram, T. Fakouhi, Pharmacology of Quercus infectoria Journal of Pharmaceutical Sciences. ,vol. 65, pp. 1791- 1794 ,(1976) , 10.1002/JPS.2600651224
Simon L Croft, Shyam Sundar, Alan H Fairlamb, None, Drug Resistance in Leishmaniasis Clinical Microbiology Reviews. ,vol. 19, pp. 111- 126 ,(2006) , 10.1128/CMR.19.1.111-126.2006
NurSaeida Baharuddin, Hasmah Abdullah, Wan NorAmilah Wan Abdul Wahab, Anti-Candida activity of Quercus infectoria gall extracts against Candida species Journal of Pharmacy and Bioallied Sciences. ,vol. 7, pp. 15- 20 ,(2015) , 10.4103/0975-7406.148742
Giuseppe Bisignano, Antonio Tomaino, Rossella Lo Cascio, Giuseppe Crisafi, Nicola Uccella, Antonella Saija, On the In‐vitro Antimicrobial Activity of Oleuropein and Hydroxytyrosol Journal of Pharmacy and Pharmacology. ,vol. 51, pp. 971- 974 ,(2010) , 10.1211/0022357991773258
Linh H. Mai, Guy G. Chabot, Philippe Grellier, Lionel Quentin, Vincent Dumontet, Cyril Poulain, Laila S. Espindola, Sylvie Michel, Hue T.B. Vo, Brigitte Deguin, Raphaël Grougnet, Antivascular and anti-parasite activities of natural and hemisynthetic flavonoids from New Caledonian Gardenia species (Rubiaceae). European Journal of Medicinal Chemistry. ,vol. 93, pp. 93- 100 ,(2015) , 10.1016/J.EJMECH.2015.01.012
Ebrahim Badparva, Farnaz Kheirandish, Farzad Ebrahimzade, None, Prevalence of intestinal parasites in Lorestan Province, West of Iran Asian Pacific Journal of Tropical Disease. ,vol. 4, ,(2014) , 10.1016/S2222-1808(14)60716-7