作者: Farnaz Kheirandish , Bahram Delfan , Hossein Mahmoudvand , Nasim Moradi , Behrouz Ezatpour
DOI: 10.1016/J.BIOPHA.2016.04.040
关键词: Chemistry 、 Quercus infectoria 、 Traditional medicine 、 Gallic acid 、 Flavonoid 、 Leishmania major 、 Biphenyl compound 、 DPPH 、 MTT assay 、 Biochemistry 、 Quercetin
摘要: Currently, there is no effective vaccine available, and chemotherapy the main approach for treatment of cutaneous leishmaniasis (CL). During recent decades, studies have demonstrated that a number plant-derived compounds may act as new therapeutic tools against leishmaniasis. This study was evaluated antileishmanial, antioxidant, cytotoxic activities Quercus infectoria Olivier (oak) extract. The total amount phenolic flavonoid measured in oak High performance liquid chromatography (HPLC) analysis also performed to determine quercetin gallic acid this plant. extract (0-80g/mL) vitro promastigote intracellular amastigote forms Leishmania major (MRHO/IR/75/ER) using MTT assay macro-phage model, respectively. Then tested on CL infected male BALB/c mice with L. order evaluate antileishmanial activity topically. Moreover, cytotoxicity effects murine macrophage cells were by assay. Antioxidative determined 2,2-diphenyl-1,1-picrylhydrazyl (DPPH) scavenging test. 57.50 1.86%, 0.0064 0.22%, findings revealed significantly (P<0.05) inhibited growth rate (IC50 12.65μg/mL) amastigotes 10.31μg/mL) dose-dependent response. In vivo assay, after 4 weeks treatment, 91.6, 66.66, 50% recovery observed treated 20, 10, 5mg/kg extract, After concentration 10 20mg/kg oak, mean diameter lesions, parasite load parasites reduced. Selectivity index greater than had cells. DPPH test radical inhibition occurred at power increasing oak. To conclude, present showed potent antioxidant extract; whereas plant toxic effect mammalian