作者: P.J.M. Bouvet , O.M.M. Bouvet
DOI: 10.1016/0923-2508(89)90085-5
关键词: Carbon source 、 Paper chromatography 、 Acinetobacter species 、 Glucose dehydrogenase activity 、 Microbiology 、 Pyrroloquinoline quinone 、 Glucose dehydrogenase 、 Biology 、 Cellulose 、 Acinetobacter 、 Biochemistry
摘要: Abstract A study of D-glucose oxidation by Acinetobacter species was carried out. Glucose-oxidizing strains were found distributed among almost all species. 14 C-glucose kinetics non-proliferating cells with separation products ( C-gluconate) DEAE- cellulose paper chromatography studied. Inhibition glucose dehydrogenase (GDH) activity 11 carbohydrates (mono- and disaccharides) determination the kinetic parameters showed that due to action membrane-bound GDH (inactive in vivo on disaccharides). On basis inhibition patterns obtained, two groups individualized. The first group (identified as A. calcoaceticus, baumannii, lwoffii, johnsonii 3, 9, 10 11) a greater affinity for than second haemolyticus, junii 6 12). Restoration after addition pyrroloquinoline quinone (PQQ) studied 187 previously unable oxidize glucose. 150 out 166 identified johnsonii, lwoffii 12 restored. Eighteen 21 haemolyticus produce acid from PQQ. Our results confirm former taxonomic sheme genus (2 differing only oxidation) is untenable that, accordingly, identification at level must be performed using more modern methods, i.e. carbon source utilization tests.