Cytogenetic effects of propyl gallate in CHO-K1 cells.

摘要: We investigated whether propyl gallate (PG) can induce sister-chromatid exchanges (SCEs) and chromosomal aberrations (CAs) in CHO-K1 cells. In the absence of an exogeneous metabolizing system, treatments with 0.25-1.5mM PG plugged flasks for 3h resulted increases SCEs, CAs, endoreduplications (ERDs), which were followed by increase percentage cells showing cell-cycle delay. At end treatment, a decrease concentration production dimer ellagic acid (EA) medium detected, indicating that had autoxidized. EA, oxide PG, was not genotoxic even at 0.3mM, maximum soluble medium. Several oxygen radical scavengers (superoxide dismutase (SOD), catalase, glutathione o-phenanthroline (OP)) inhibitor catalase activity (3-amino-1,2,4-triazole (AT)), did significantly influence genotoxicity. When autoxidation suppressed low pH (6.8) or 5% CO(2) atmosphere, delay intensified induction SCEs CAs occurred lowest dose (0.1mM). (0.5mM) assayed presence S9 (1.5-9%), gallic (GA), metabolite generated direct proportion to concentration, while effects varied inversely levels over 3%. GA also autoxidized >or=0.5mM it induced SCEs. Both AT inhibited cell proliferation, H(2)O(2) participated effects. conclusion, ERDs, oxides, metabolites oxygen-free radicals during treatment are partly responsible these