2010 Special Issue: Fluorescent pipettes for optically targeted patch-clamp recordings
作者： Daisuke Ishikawa , Naoya Takahashi , Takuya Sasaki , Atsushi Usami , Norio Matsuki
DOI: 10.1016/J.NEUNET.2010.02.004
关键词: Pipette 、 Biomedical engineering 、 Biological neural network 、 Microscopy 、 Patch clamp 、 Neuron 、 Materials science 、 Confocal microscopy 、 Fluorescence microscope 、 Microscope
摘要: Targeted patch-clamp recordings are a promising technique that can directly address the physiological properties of specific neuron embedded in neuronal network. Typically, neurons visualized through fluorescent dyes or proteins with fluorescence microscopy. After switching to transmitted light microscopy, interest re-identified and visually approached situ pipettes. Here we introduce simpler method for targeting. With fluorophore-coated pipettes, fluorescently labeled pipette tips simultaneously imaged at same wavelength microscope field, so even their neurites targeted without suffering from chromatic aberration mechanical complication optics. We did not find coated fluorophores affected electric pipettes neurons. The novel will be wildly available micromanipulation under online visual control.
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sci-hub.se 参考文章(21)
Kazuo Kitamura, Benjamin Judkewitz, Masanobu Kano, Winfried Denk, Michael Häusser, Targeted patch−clamp recordings and single−cell electroporation of unlabeled neurons in vivo Nature Methods. ,vol. 5, pp. 61- 67 ,(2008) , 10.1038/NMETH1150
G. J. Stuart, H. U. Dodt, B. Sakmann, Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy Pflügers Archiv: European Journal of Physiology. ,vol. 423, pp. 511- 518 ,(1993) , 10.1007/BF00374949
M. Galarreta, S. Hestrin, Electrical and chemical synapses among parvalbumin fast-spiking GABAergic interneurons in adult mouse neocortex Proceedings of the National Academy of Sciences of the United States of America. ,vol. 99, pp. 12438- 12443 ,(2002) , 10.1073/PNAS.192159599
Junya Ichikawa, Naoya Takahashi, Atsushi Usami, Maki K. Yamada, Norio Matsuki, Yuji Ikegaya, Ryuta Koyama, Rieko Muramatsu, Takuya Sasaki, Rie Kimura, Chihiro Ueyama, Makoto Tamura, Naohiro Tamura, A Low-Cost Method for Brain Slice Cultures Journal of Pharmacological Sciences. ,vol. 104, pp. 191- 194 ,(2007) , 10.1254/JPHS.SC0070119
H. -U. Dodt, A. Frick, K. Kampe, W. Zieglgänsberger, NMDA and AMPA receptors on neocortical neurons are differentially distributed European Journal of Neuroscience. ,vol. 10, pp. 3351- 3357 ,(1998) , 10.1046/J.1460-9568.1998.00338.X
Friedrich Metzger, Vez Repunte-Canonigo, Shinichi Matsushita, Walther Akemann, Javier Diez-Garcia, Chi Shun Ho, Takuji Iwasato, Pedro Grandes, Shigeyoshi Itohara, Rolf H. Joho, Thomas Knöpfel, Transgenic mice expressing a pH and Cl- sensing yellow-fluorescent protein under the control of a potassium channel promoter. European Journal of Neuroscience. ,vol. 15, pp. 40- 50 ,(2002) , 10.1046/J.0953-816X.2001.01837.X
Hans-Ulrich Dodt, Walter Zieglgänsberger, Visualizing unstained neurons in living brain slices by infrared DIC-videomicroscopy Brain Research. ,vol. 537, pp. 333- 336 ,(1990) , 10.1016/0006-8993(90)90380-T
Tetsuichiro Saito, Norio Nakatsuji, Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. Developmental Biology. ,vol. 240, pp. 237- 246 ,(2001) , 10.1006/DBIO.2001.0439
Troy W Margrie, Axel H Meyer, Antonio Caputi, Hannah Monyer, Mazahir T Hasan, Andreas T Schaefer, Winfried Denk, Michael Brecht, Targeted whole−cell recordings in the mammalian brain in vivo Neuron. ,vol. 39, pp. 911- 918 ,(2003) , 10.1016/J.NEURON.2003.08.012
T. Dittgen, A. Nimmerjahn, S. Komai, P. Licznerski, J. Waters, T. W. Margrie, F. Helmchen, W. Denk, M. Brecht, P. Osten, Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo Proceedings of the National Academy of Sciences of the United States of America. ,vol. 101, pp. 18206- 18211 ,(2004) , 10.1073/PNAS.0407976101