High-speed multineuron calcium imaging using Nipkow-type confocal microscopy.
作者： Naoya Takahashi , Shigeyuki Oba , Naoto Yukinawa , Sakiko Ujita , Mika Mizunuma
DOI: 10.1002/0471142301.NS0214S57
关键词: Laser illumination 、 Calcium imaging 、 Biomedical engineering 、 Confocal 、 Confocal microscopy 、 Cellular resolution 、 Frame rate 、 Laser beams 、 Microscopy 、 Nanotechnology 、 Chemistry
摘要: Conventional confocal and two-photon microscopy scan the field of view sequentially with single-point laser illumination. This raster-scanning method constrains video speeds to tens frames per second, which are too slow capture temporal patterns fast electrical events initiated by neurons. Nipkow-type spinning-disk resolves this problem use multiple beams. We describe experimental procedures for functional multineuron calcium imaging (fMCI) based on Nipkow-disk microscopy, enables us monitor activities hundreds neurons en masse at a cellular resolution up 2000 fps. Curr. Protoc. Neurosci. 57:2.14.1-2.14.10. © 2011 John Wiley & Sons, Inc. Keywords: imaging; microscopy; calcium; neuron; spike
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