Role of inducible nitric oxide synthase expression in aberrant crypt foci-adenoma-carcinoma sequence

摘要: AIM: To investigate the expression of inducible nitric oxide synthase (iNOS) in aberrant crypt foci (ACF) -adenoma-carcinoma sequence and its relation with tumor cell apoptosis, proliferation angiogenesis. METHODS: The iNOS, proliferating nuclear antigen (PCNA) microvessel density (MVD) different stages colorectal cancer were studied by immunohistochemical method from 30 normal tissues, nonhyperplastic ACF, hyperplastic dysplastic adenomas 60 carcinomas. apoptotic cells detected terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) using an Apop Tag situ detection kit. RESULTS: immunoreactivity iNOS significantly increased transition ACF to ACF. This was associated a significant decrease index (AI) (0.73 ± 0.37 vs 0.61 0.35, P < 0.05) increases PCNA (LI) (27.3 2.80 40.3 3.11, 0.01) (55 11.5 70 13.2, 0.01). low levels positively correlated PCNA-LI (r = 0.812, MVD 0.863, during mucosa high AI 0.901, after adenoma carcinoma. CONCLUSION: results suggest that may be crucial step ACF-adenoma-carcinoma sequence, which plays important role regulating angiogenesis.