Roles of the TRAPP-II Complex and the Exocyst in Membrane Deposition during Fission Yeast Cytokinesis
作者： Ning Wang , I-Ju Lee , Galen Rask , Jian-Qiu Wu
DOI: 10.1371/JOURNAL.PBIO.1002437
关键词: Exocyst 、 Golgi apparatus 、 Endocytic cycle 、 Biology 、 Septin 、 Cytokinesis 、 Exocytosis 、 Vesicle 、 Cleavage furrow 、 Cell biology 、 General Biochemistry, Genetics and Molecular Biology 、 General Immunology and Microbiology 、 General Neuroscience 、 General Agricultural and Biological Sciences
摘要: The cleavage-furrow tip adjacent to the actomyosin contractile ring is believed be predominant site for plasma-membrane insertion through exocyst-tethered vesicles during cytokinesis. Here we found that most secretory are delivered by myosin-V on linear actin cables in fission yeast Surprisingly, tracking individual exocytic and endocytic events, with new membrane deposited cleavage furrow relatively evenly contractile-ring constriction, but rim of main endocytosis. Fusion plasma requires vesicle tethers. Our data suggest transport particle protein II (TRAPP-II) complex Rab11 GTPase Ypt3 help tether or tubulovesicular structures along while exocyst tethers at division plane. We conclude TRAPP-II have distinct localizations site, both important expansion exocytosis
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sci-hub.se 参考文章(131)
Gerald R. Prescott, Oforiwa A. Gorleku, Jennifer Greaves, Luke H. Chamberlain, Palmitoylation of the synaptic vesicle fusion machinery Journal of Neurochemistry. ,vol. 110, pp. 1135- 1149 ,(2009) , 10.1111/J.1471-4159.2009.06205.X
D. R. TerBush, T. Maurice, D. Roth, P. Novick, The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. The EMBO Journal. ,vol. 15, pp. 6483- 6494 ,(1996) , 10.1002/J.1460-2075.1996.TB01039.X
Thomas H. Giddings, Eileen T. O'Toole, Mary Morphew, David N. Mastronarde, J.Richard McIntosh, Mark Winey, Using rapid freeze and freeze-substitution for the preparation of yeast cells for electron microscopy and three-dimensional analysis Methods in Cell Biology. ,vol. 67, pp. 27- 42 ,(2001) , 10.1016/S0091-679X(01)67003-1
Geert van den Bogaart, Thorsten Lang, Reinhard Jahn, Microdomains of SNARE Proteins in the Plasma Membrane Functional Organization of Vertebrate Plasma Membrane. ,vol. 72, pp. 193- 230 ,(2013) , 10.1016/B978-0-12-417027-8.00006-4
Isabelle Jourdain, Hannah C. Dooley, Takashi Toda, Fission yeast sec3 bridges the exocyst complex to the actin cytoskeleton. Traffic. ,vol. 13, pp. 1481- 1495 ,(2012) , 10.1111/J.1600-0854.2012.01408.X
Margaret R. Heider, Mary Munson, Exorcising the Exocyst Complex Traffic. ,vol. 13, pp. 898- 907 ,(2012) , 10.1111/J.1600-0854.2012.01353.X
Kurt J. Amann, Thomas D. Pollard, The Arp2/3 complex nucleates actin filament branches from the sides of pre-existing filaments Nature Cell Biology. ,vol. 3, pp. 306- 310 ,(2001) , 10.1038/35060104
David Taussig, Zhanna Lipatova, Jane J Kim, XuiQi Zhang, Nava Segev, None, Trs20 is required for TRAPP II assembly. Traffic. ,vol. 14, pp. 678- 690 ,(2013) , 10.1111/TRA.12065
Reshma Davidson, Yajun Liu, Kenneth S. Gerien, Jian-Qiu Wu, Real-Time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells Methods in Molecular Biology. ,vol. 1369, pp. 9- 23 ,(2016) , 10.1007/978-1-4939-3145-3_2
Kirk W. Donovan, Anthony Bretscher, Tracking individual secretory vesicles during exocytosis reveals an ordered and regulated process Journal of Cell Biology. ,vol. 210, pp. 181- 189 ,(2015) , 10.1083/JCB.201501118