Real-Time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells
作者： Reshma Davidson , Yajun Liu , Kenneth S. Gerien , Jian-Qiu Wu
DOI: 10.1007/978-1-4939-3145-3_2
关键词:
摘要: Single-cell microscopy provides a powerful tool to visualize cellular and subcellular processes in wild-type mutant cells by observing fluorescently tagged proteins. Here, we describe three simple methods fission yeast cells: gelatin slides, coverslip-bottom dishes, tetrad fluorescence microscopy. These imaging data analysis using free software make it possible quantify protein localization, dynamics, concentration with high spatial temporal resolution. In yeast, the actomyosin contractile ring is essential for cytokinesis. We use visualization quantification of proteins as an example demonstrate how these methods.
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