Comparative Effects of n-3, n-6 and n-9 Unsaturated Fatty Acid-Rich Diet Consumption on Lupus Nephritis, Autoantibody Production and CD4+ T Cell-Related Gene Responses in the Autoimmune NZBWF1 Mouse

摘要: Mortality from systemic lupus erythematosus (SLE), a prototypical autoimmune disease, correlates with the onset and severity of kidney glomerulonephritis. There are both preclinical clinical evidence that SLE patients may benefit consumption n-3 polyunsaturated fatty acids (PUFA) found in fish oil, but mechanisms remain unclear. Here we employed NZBWF1 mouse model to compare effects dietary lipids on glomerulonephritis after consuming: 1) PUFA-rich diet containing docosahexaenoic acid-enriched oil (DFO), 2) n-6 Western-type corn (CRN) or 3) n-9 monounsaturated acid (MUFA)-rich Mediterranean-type high oleic safflower (HOS). Elevated plasma autoantibodies, proteinuria were evident mice fed either PUFA MUFA diets, however, all three endpoints markedly attenuated consumed until 34 wk age. A focused PCR array was used relate these findings expression 84 genes associated CD4+ T cell function spleen prior nephritis. suppression autoimmunity co-occur generalized downregulation cell-related and/or at 34. These inflammatory response, antigen presentation, activation, B activation/differentiation leukocyte recruitment. Quantitative RT-PCR representative affected confirmed reduced CD80, CTLA-4, IL-10, IL-18, CCL-5, CXCR3, IL-6, TNF-α osteopontin mRNAs spleens as compared diets. Remarkably, many identified this study currently under consideration biomarkers biotherapeutic targets for other diseases.