Epidermal growth factor (EGF) receptor gene transcription. Requirement for Sp1 and an EGF receptor-specific factor.
作者： R Kageyama , G T Merlino , I Pastan
DOI: 10.1016/S0021-9258(18)68790-3
关键词: Cell surface receptor 、 Sp1 transcription factor 、 Epidermoid carcinoma 、 Growth factor receptor 、 Growth factor receptor inhibitor 、 Molecular biology 、 Heparin-binding EGF-like growth factor 、 Transcription factor 、 Epidermal growth factor 、 Biology 、 Cell biology 、 Biochemistry
摘要: We have studied in vitro transcription of the human epidermal growth factor (EGF) receptor proto-oncogene using nuclear extracts A431 epidermoid carcinoma cells, which overproduce EGF receptor. With system we found that Sp1 and other trans-acting factors bound to promoter regions are required for maximal expression. Fractionation showed a DEAE-Sepharose fraction (BA) contained novel factor, specifically stimulated 5- 10-fold. The molecular mass native form is about 270-kDa based on its migration Sephacryl S-300. This may activate through weak or indirect interaction with DNA template.
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