The formation of golgi stacks from vesiculated golgi membranes requires two distinct fusion events

摘要: Abstract We have reconstituted the fusion and assembly of vesiculated Golgi membranes (VGMs) into functionally active stacks cisternae. A kinetic analysis this process revealed that highly dispersed VGMs 60–90 nm diameter first fuse to form larger vesicles 200–300 are clustered together. These then tubular elements short cisternae, which finally assemble now provide evidence sequential stack formation from reflects two distinct processes: event is N-ethylmaleimide (NEM)-sensitive factor (NSF) dependent, second requires an NSF-like NEM-sensitive ATPase called p97. Interestingly, while earliest steps in share some similarities with events catalyzing transport its target membrane, neither GTPγS nor Rab-GDI, inhibitors vesicular protein traffic, inhibit formation.