Induction of specific histamine release from basophils with purified natural and recombinant birch pollen allergens

摘要: Abstract As much as 15% of the population in industrialized countries suffers from type I allergic symptoms (rhinitis, conjunctivitis, and bronchial asthma). One approach toward this disease involves production recombinant allergens Escherichia coli their purification for diagnostic therapeutic purposes. In study we compared IgE-binding capacity natural birth with functional ability to release histamine patients' basophils via cross-linking high-affinity Fc ϵ -receptors. The well pollen-derived Bet v birch profilin ( II) were purified tested parallel on IgE immunoblots vitro tests n = 21). We observed an exellent correlation between allergen-induced pollen RAST r 0.881, p