Restimulation properties of cloned alloactivated lymphocytes: Detection of a novel type of HLA-D/DR region determinant and allelic subtypes for HLA-Dw3 using cloned reagents
作者： G. Pawelec , A. Rehbein , P. Kahle , C. Müller , P. Wernet
DOI: 10.1016/0198-8859(81)90033-1
关键词: Epitope 、 Antigen 、 Lymphocyte 、 Priming (immunology) 、 Human leukocyte antigen 、 Pan-T antigens 、 Molecular biology 、 Growth factor 、 Biology 、 Typing
摘要: Lymphocytes alloactivated by Dw3 homozygous typing cells were cloned the method of limiting dilution and cultured for prolonged periods using T-cell growth factor irradiated pooled leukocytes (as feeder cells). Restimulation specificity two clones functioning as primed lymphocyte reagents was investigated in panel family studies. Cells from one (12-2) always specifically stimulated HLA-Dw3 antigens shared between original priming stimulating cells. In an informative KOH, however, this clone seemed to detect a split cluster. other (12-8) failed respond expressed or cells; rather, restimulation be associated with expression Dw4 antigens. Family segregation analysis did not support conclusion since products segregated three Dw3-bearing haplotypes none Dw4-bearing haplotypes. This suggested that 12-8 may responsive different those detected
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