Interaction between brain natriuretic peptide and atrial natriuretic peptide in caecal circular smooth muscle cells.

摘要: Guinea pig caecal circular smooth muscle cells were used to determine whether brain natriuretic peptide (BNP) can inhibit the contractile response produced by cholecystokinin-octapeptide (CCK-8). In addition, we examined effect of an inhibitor cAMP-dependent protein kinase, particulate or soluble guanylate cyclase, atrial (ANP) antagonist (ANP 1-11), and selective receptor protection on BNP-induced relaxation these cells. The BNP cAMP formation was also examined. inhibited CCK-8 in a dose-response manner, with IC50 value 8.5 nM, stimulated production cAMP. kinase cyclase significantly BNP. contrast, did not have any significant ANP 1-11 but partially where binding sites protected completely preserved inhibitory ANP, both This study demonstrates that induces via coupled distinct adenylate cyclase.