The use of mitochondrial DNA polymorphisms to distinguish commercial cultivars of the broad bean, Vicia faba L.
作者： C. D. Scallan , M. A. Harmey
DOI: 10.1007/BF00023749
关键词: Southern blot 、 Genetics 、 Restriction enzyme 、 Nucleic acid 、 Ethidium bromide 、 Molecular biology 、 Vicia faba 、 Biology 、 Genetic variability 、 Restriction fragment length polymorphism 、 Mitochondrial DNA 、 Agronomy and Crop Science 、 Plant science 、 Horticulture
摘要: Restriction fragment length polymorphisms (RFLPs) have been used to detect mitochondrial DNA (mtDNA) variation among 9 commercial cultivars of Vicia faba L. The was initially digested with 8 restriction endonucleases revealing complex banding patterns on ethidium bromide (EtBr) stained gels. However, no RFLPs were visualised from these profiles. Southern hybridisation using total mtDNA as a probe against the same enzymes revealed limited number which allowed be consistently distinguished into two main cytoplasmic types. 23 random clones covering 56 kb genome considerable levels polymorphisms. Of analysed, 12 detected at least 22 3 analysed. These analysed in this study 6 separate groups. Most
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