S phase-specific synthesis of dihydrofolate reductase in Chinese hamster ovary cells.
作者： B. D. Mariani , D. L. Slate , R. T. Schimke
关键词: Dihydrofolate reductase 、 DNA synthesis 、 Chinese hamster ovary cell 、 Oxidoreductase 、 Molecular biology 、 Mitosis 、 Biochemistry 、 Biology 、 DNA 、 DNA replication 、 Cell cycle
摘要: We investigated the cell cycle modulation of dihydrofolate reductase (DHFR; tetrahydrofolate dehydrogenase, 7,8-dihydroxyfolate:NADP+ oxidoreductase, EC 1.5.1.3) levels in methotrexate-resistant Chinese hamster ovary cells synchronized by mitotic selection. DNA content and DHFR concentration were analyzed throughout standard biochemical techniques double fluorescence staining utilizing fluorescence-activated sorter. found an S phase-specific period biosynthetic activity. Commencing within hour 2 phase continuing duration phase, there is a 90% increase specific This results from approximately 2.5-fold level DHFR, while total soluble protein increases 50% during same period. result new synthesis molecules initiated after physiologically committed to replication. activity through phage parallels increasing rate [3H]thymidine incorporation interval. The maximum peak coincident with synthesis, both activities occurring bulk replication last stages 6.5-hr phase.
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sci-hub.se 参考文章(22)
J.W. Gray, P. Coffino, [19] Cell cycle analysis by flow cytometry Methods in Enzymology. ,vol. 58, pp. 233- 248 ,(1979) , 10.1016/S0076-6879(79)58140-3
R E Kellems, R T Schimke, J R Bertino, F W Alt, Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells. Journal of Biological Chemistry. ,vol. 253, pp. 1357- 1370 ,(1978) , 10.1016/S0021-9258(17)34875-5
L.G. Navalgund, C. Rossana, A.J. Muench, L.F. Johnson, Cell cycle regulation of thymidylate synthetase gene expression in cultured mouse fibroblasts. Journal of Biological Chemistry. ,vol. 255, pp. 7386- 7390 ,(1980) , 10.1016/S0021-9258(20)79715-2
F W Alt, R E Kellems, R T Schimke, Synthesis and degradation of folate reductase in sensitive and methotrexate-resistant lines of S-180 cells. Journal of Biological Chemistry. ,vol. 251, pp. 3063- 3074 ,(1976) , 10.1016/S0021-9258(17)33500-7
Sandra Murphree, E. Stubblefield, E.Colleen Moore, Synchronized mammalian cell cultures Experimental Cell Research. ,vol. 58, pp. 118- 124 ,(1969) , 10.1016/0014-4827(69)90121-9
R. T. Schimke, R. J. Kaufman, Joseph Bertino, Quantitation of dihydrofolate reductase in individual parental and methotrexate-resistant murine cells. Use of a fluorescence activated cell sorter. Journal of Biological Chemistry. ,vol. 253, pp. 5852- 5860 ,(1978) , 10.1016/S0021-9258(17)30347-2
Abigail H. Conrad, Thymidylate Synthetase Activity in Cultured Mammalian Cells Journal of Biological Chemistry. ,vol. 246, pp. 1318- 1323 ,(1971) , 10.1016/S0021-9258(19)76977-4
R.E. Kellems, V.B. Morhenn, E.A. Pfendt, F.W. Alt, R.T. Schimke, Polyoma virus and cyclic AMP-mediated control of dihydrofolate reductase mRNA abundance in methotrexate-resistant mouse fibroblasts. Journal of Biological Chemistry. ,vol. 254, pp. 309- 318 ,(1979) , 10.1016/S0021-9258(17)37919-X
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
W. Kersten, H. Kersten, W. Szybalski, M. Fiandt, Physicochemical Properties of Complexes between Deoxyribonucleic Acid and Antibiotics Which Affect Ribonucleic Acid Synthesis (Actinomycin, Daunomycin, Cinerubin, Nogalamycin, Chromomycin, Mithramycin, and Olivomycin)* Biochemistry. ,vol. 5, pp. 236- 244 ,(1966) , 10.1021/BI00865A031