Ex vivo Sensitivity Profile of Plasmodium falciparum Clinical Isolates to a Panel of Antimalarial Drugs in Ghana 13 Years After National Policy Change.

摘要: Purpose Malaria continues to be a major health issue globally with almost 85% of the global burden and deaths borne by sub-Saharan Africa India. Although current artemisinin derived combination therapies in Ghana are still efficacious against Plasmodium falciparum (Pf) parasite, compounding evidence amodiaquine resistance establish need for full, up-to-date understanding monitoring antimalarial provide planning control strategies. Materials Methods The study was cross-sectional conducted during peak malaria transmission seasons 2015, 2016, 2017 two ecological zones Ghana. Study participants included children aged 6 months 14 years. Using ex vivo 4,6-diamidino-2-phenylindole (DAPI) drug sensitivity assay, 330 Pf isolates were used investigate susceptibility five drugs: chloroquine (CQ), (AMD) dihydroartemisinin (DHA), artesunate (ART) mefloquine (MFQ). Results pooled geometric mean IC50S (GMIC50) drugs parasites from Cape Coast Begoro 15.5, 42.4, 18.9, 4.6 27.3nM CQ, AMD, DHA, ART, MFQ, respectively. GMIC50 values CQ (p<0.001), ART (p<0.011) DHA (p<0.018) significantly higher as compared isolates. However, estimates MFQ (p<0.022) Positive correlations found between each pair weakest (r = 0.34;p<0.001), strongest =0.66; p<0.001). Conclusion showed reduced sensitivities three (AMD, MFQ) out assessed. also demonstrated continual return chloroquine-sensitive after 13 years its withdrawal first-line treatment uncomplicated DAPI assay is reliable method assessing field under settings.