Dietary Sulforaphane-Rich Broccoli Sprouts Reduce Colonization and Attenuate Gastritis in Helicobacter pylori–Infected Mice and Humans

摘要: The isothiocyanate sulforaphane [SF; 1-isothiocyanato-4(R)-methylsulfinylbutane] is abundant in broccoli sprouts the form of its glucosinolate precursor (glucoraphanin). SF powerfully bactericidal against Helicobacter pylori infections, which are strongly associated with worldwide pandemic gastric cancer. Oral treatment SF-rich C57BL/6 female mice infected H. Sydney strain 1 and maintained on a high-salt (7.5% NaCl) diet reduced bacterial colonization, attenuated mucosal expression tumor necrosis factor-alpha interleukin-1beta, mitigated corpus inflammation, prevented high salt-induced atrophy. This therapeutic effect was not observed nrf2 gene deleted, implicating important role Nrf2-dependent antioxidant anti-inflammatory proteins SF-dependent protection. Forty-eight pylori-infected patients were randomly assigned to feeding (70 g/d; containing 420 micromol precursor) for 8 weeks or consumption an equal weight alfalfa (not SF) as placebo. Intervention sprouts, but placebo, decreased levels urease measured by urea breath test stool antigen (both biomarkers colonization) serum pepsinogens I II (biomarkers inflammation). Values recovered their original 2 months after discontinued. Daily intake sulforaphane-rich reduces colonization improves sequelae infection humans. seems enhance chemoprotection mucosa pylori-induced oxidative stress.