作者: Linshan Wang , Weilin Li , Li Ma , Jian Chen , Han Lü
DOI: 10.1016/J.INDCROP.2016.01.050
关键词: Secondary metabolism 、 Botany 、 Glucoside 、 Limonium 、 Chalcone synthase 、 Vanillic acid 、 Epigallocatechin gallate 、 Gallic acid 、 Halophyte 、 Biology
摘要: Abstract In order to understand the response mode of main chemical compounds salt-stress, compositions in leaves and roots NaCl-treated Limonium bicolor (Bag.) Kuntze, a medicinal halophytic plant with efficacy blood enriching hemostasis, were determined by UPLC-Q-TOF-MS2 HPLC, compared control, expression analysis chalcone synthase (CHS) gene under salt-stress was also carried out quantitative real-time PCR. As result, eleven constituents including triethyl citrate (1), gallic acid (2), vanillic (3), derivative (4), acid-hexose (5), N-(2-carboxyethyl) iminodiacetic (6), epigallocatechin gallate (7), myricetin-3-O-glucoside/galactoside (8), myricetin-3-O-rhamnoside (9), quercetin-3-O-rhamnoside (10) myricetin-3-O-(O-galloyl)-rhamnoside (11) identified, showed that NaCl-stress can induce accumulation changes these compounds. Among them, compound 9 highest abundance both leaf root L. bicolor, which be markedly enhanced NaCl-stress. CHS sensitive NaCl-stress, its level up-regulated while inhibited These results will provide references for further research directional induction active ingredients salt mechanism secondary metabolism halophyte bicolor.