Progressive Structural Transitions within Mu Transpositional Complexes

摘要: Assembly of the Mu transpososome is dependent on specific binding sites for MuA transposase near ends phage genome. also contacts terminal nucleotides but only upon assembly, and base-specific recognition critical assembly. We show that lacking 5 bp can form transpososomes, while longer DNA substrates with mutated cannot. The impact mutations be suppressed by base mismatches end Mu. Deletion flanking strands or mutation has differential effects cleavage strand transfer reactions. These results control assembly activation transpososomes influencing conformational changes around active site.