3D reconstruction of the Mu transposase and the Type 1 transpososome: a structural framework for Mu DNA transposition
作者： J. F. Yuan
DOI: 10.1101/GAD.1291405
关键词: Genetics 、 Biophysics 、 DNA 、 DNA transposition 、 Biochemical function 、 Transposase 、 Structural framework 、 Transposition (music) 、 Transposable element 、 Mu transposase 、 Biology
摘要: Mu DNA transposition proceeds through a series of higher-order nucleoprotein complexes called transpososomes. The structural core the transpososome is tetramer transposase, A, bound to two transposon ends. High-resolution analysis intact transposase and has not been successful date. Here we report structure A at 16-A Type 1 34-A resolution, by 3D reconstruction images obtained scanning transmission electron microscopy (STEM) cryo-temperatures. Electron spectroscopic imaging (ESI) DNA-phosphorus was performed in conjunction with investigation derive path define DNA-binding surface transposase. Our model fits well accumulated biochemical literature for this intricate system, lays foundation function, including catalysis trans complex circuit macromolecular interactions underlying transposition.
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